Wiki
Welcome to the Biooptics Wiki
Then you are at the right place! The purpose of this wiki is to provide aid with setting up any of our systems for imaging, be it basic or complex.
In the sidebar on the right hand side, you can navigate the wiki - under
You will find guides for startup/shutdown, how to set up incubation and environmental control and how to navigate the various software platforms.
The Wiki is continuously expanded and updated, so if you have any input, find errors, or have suggestions for material you are missing, send us an E-Mail via the usual channels.
Aside from providing technical information, manuals and guides for our instruments at the facility, you can also find useful links & tools. Check out interactive Java tutorials, from Evident (formally Olympus) - these applets visualize some central technical concepts around bioimaging.
At the bottom of the sidebar, you will also find useful links to webpages you might need during/ in preparation for an experiment. Click on the SVI logo to be directed to the Nyquist calculator or on the FPbase logo to be directed there. Information on these sites can be found here.
Our Instruments
Laser Scanning Microscopes
LSM 980
Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives.
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LSM 900
Inverse confocal microscope with two VSDs (Variable Secondary Dichroic) allowing for finer spectral adjustment and spectral Airyscan imaging. Two highly sensitive GaAsP-PMTs for high sensitivity + Airyscan 2 detector (32 channel GaAsP) for super-resolution imaging or high-speed acquisition (Multiplex mode with up to 4x parallelisation of detection), transmitted light PMT. AI Sample finder for automated sample recognition. Motorized XY stage with Z-Piezo top plate, fully incubated. Widefield illumination: 4-channel Zeiss Colibri LED, halogen lamp, Axiocam 305 for sample overviews. (room 1.223)
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LSM 700
Installed: July 2014. Inverse point scanning confocal microscope with 2 PMT channels. Variable dichroic crystal configuration also allowing (slow) spectral imaging), transmitted light PMT and motorized stage. Widefield illumination: halogen (transmitted) and CoolLed pE-2 LED (reflected). ZEN 2012 acquisition software including "tiling & stitching" macro for larger overview imaging.
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Super-resolution Microscopes
Elyra 7
Inverse super-resolution microscope for lattice SIM, 3D SMLM (PALM/STORM), TIRF and laser widefield imaging. Apotome mode for optical sectioning of thick specimen. Dual-camera port with 2 sCMOS cameras for fast acquisitions. Lasers to image dyes from blue to far red. Multi-positioning (Piezo-driven XY stage with Z-Piezo insert) and hardware autofocus. Environmental box for live imaging with temperature and CO2 control. Widefield illumination: Excelitas Xylis (white LED), halogen lamp. Processing PC for offline image processing (room 1.723)
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STED
Upright point scanning microscope with 2 avalanche photodiode detectors for dual-channel 2D STED (orange, dark red) or conventional confocal imaging of 3 dyes (green, orange and dark red). Piezo-driven z-focus for 3D acquisition, manual XY stage for standard slides. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.723)
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High-througput Microscopes
Celldiscoverer 7
System for high-throughput live imaging and screening. For multiwell plates (glass or plastic bottom), dishes and chamber slides. Hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), sCMOS camera, 7-color LED for fluorescence, IR-LED for transmitted light and phase gradient contrast, temperature/CO2 control. Python interface for real-time image analysis and custom workflows (room 1.320)
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Spinning Disk Microscopes
Live Spinning Disk
Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318).
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Spinning Disk 1
Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223)
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Widefield Microscopes
Olympus CellSens
Inverse widefield microscope unit with solid state LED light source and filter sets to image all conventional dyes. 3-color TIRF and single-point 405 nm FRAP option. Equipped with a 4 Megapixel sCMOS camera for fast high-resolution imaging, sensitive EM-CCD for TIRF and low-light applications, or color CMOS camera. Motorized stage for 5D-data acquisition, full environmental control for long-term live imaging. Microfluidics device for mammalian cells, yeast and bacteria (room 1.223)
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DeltaVision Ultra
Inverse widefield microscope in Sedat setup equipped with a 4-Megapixel sCMOS camera for fast fluorescence and brightfield imaging. 7-color solid state light source for fluorescence imaging with Köhler and critical illumination, respectively, white LED for brightfield, DIC optics. Motorized XY stage, hardware autofocus and environmental chamber for temperature and CO2 control, onboard deconvolution (room 1.320)
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Applicability of Microscopes
| usage | STED | Live-Spinning-Disk | Spinning-Disk-I | LSM-980 | LSM-900 | LSM-700 | Elyra-7 | CD-7 | CellSens | DV Ultra |
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| Resolution | +++++ | ++ | ++ | ++++ | ++++ | +++ | +++++ | ++ | +++ | ++ |
| Live-Imaging | - | +++++ | + | +++ | ++++ | + | ++++ | +++++ | ++ | +++++ |
| Thick Samples | + | +++ | +++ | +++ | +++ | +++ | ++ | ++ | + | +++++ |
| High Throughput Imaging | + | ++++ | ++++ | ++ | ++ | + | + | +++++ | + | + |
| 3D-Imaging | - | +++ | +++ | +++++ | +++++ | ++++ | +++ | + | + | + |
| Acquisition Speed | ++ | +++++ | +++++ | ++++ | +++ | ++ | +++ | +++++ | + | ++ |
| FOV | variable | +++++ | +++++ | variable | variable | variable | ++++ | ++++ | + | +++ |
Manuals
| Live Spinning Disk | Spinning Disk | STEDYCON |
|---|---|---|
| Technical Specifications | Technical Specifications | Technical Specifications |
| Startup/Shutdown Guide | Startup/Shutdown Guide | Startup/Shutdown Guide |
| Sample Preparation Tips | ||
| User Manual |
| LSM-980 | LSM-900 | LSM-700 |
|---|---|---|
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Technical Specifications |
Technical Specifications |
Technical Specifications |
| Startup/Shutdown Guide | Startup/Shutdown Guide | Startup/Shutdown Guide |
| Elyra | CellSens | Cell Discoverer |
|---|---|---|
| Technical Specifications | Technical Specifications | Technical Specifications |
| Startup/Shutdown Guide | Startup/Shutdown Guide | Startup/Shutdown Guide |
| Temperature & CO2 |