- By default, the system should be running at 37°C. Do not switch off the controller after your session. If you need lower temperatures, please contact the person signed in after you far in advance and ask for their permission, as the temperature will need some time to increase again after your experiment.
- Open the main valve of the CO2 tank by turning it counter clockwise for 1 round (no need to rotate it up to the stop). Close it clockwise once you are done. Don't touch the other valves!
- Power on the CO2 controller (power switch on its back side).
- Place the CO2 cover on the CO2 insert and make sure that the humidifier bottle is filled with enough distilled water (>50 mL; the bottle can be disconnected with two luer connectors). To replenish water (deionized, autoclaved water only), contact the facility staff!
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- Acquire wavelengths at each Z is preferred for z-stacks of rapidly moving objects (shortest possible interval between different channels).
- Z Series for each wavelength is overall faster (requires less movements of the slow filter wheel).
- File format: in the top menu, select Configure → System. In the dialog popping up, select the Acquisition tab. Under Save File, select file format:
Metamorph STK is recommended for all kinds of multidimensional acquisitions.
OME Tiff is recommended single-channel FRAP experiments. It stores FRAP regions in the metadata. Check the auto checkbox. VisiView will automatically select the proper OME Tiff subtype (32 bit as *.ome.tif, 64 bit version as *.ome.btf).
