Instruments/Live-Spinning-Disk.md

@@ -138,30 +138,14 @@ OME Tiff is recommended single-channel FRAP experiments. It stores FRAP regions
 
 ![image](uploads/123007a4befb2d29a1dc069e67d12646/image.png)
 
-## Shutdown
-- On the microscope, press the ESC button to bring the objective in a safe position.
-- If using CO2 control:
-   - Turn off the CO2 flow on the CO2 controller.
-   - Power off the controller.
-   - Close the main valve of the CO2 tank.
-- Remove your sample.
-- Clean all used immersion objectives with lens cleaning paper.
-
-![Cleaning](uploads/a8aa76b103e7e28022be55e048ecc5b0/Cleaning.jpg){width=30%}
+## Image Acquisition
 
-- Exit VisiView.
-- When leaving the system for the next user: sign out from Windows.
-- Full shutdown:
-   - Shut down the computer.
-   - Swith off all laser keys (4).
-   - Power off the power strips in reverse order (3, 2, 1).
-
-## Hardware Autofocus
+### Hardware Autofocus
 
 The hardware autofocus permits focus stabilization under non-bleaching conditions. It uses backreflection of grid pattern generated with a weak LED (850 nm) to measure the distance between the objective and the cover glass and to keep it constant. An aqueous medium (with a refractive index close to that of water) is required. It cannot be used with glycerol-based or hardening mounting media.
 Objects above the cover glass/medium interface are not monitored. If objects of interest can change their focus position (e.g., cells rounding up during mitosis), the software autofocus should be used.
 
-### A. Continuous mode (constant offset)
+#### A. Continuous mode (constant offset)
 
 This mode is convenient to keep the focus at a contant distance above the cover glass while moving the XY stage across large areas of the sample.
 1. Click the button "HW-AF Dialog" in the toolbar on the right side of the application. Alternatively, the dialog can be opened with the menu command Configure → Autofocus.
@@ -172,7 +156,7 @@ This mode is convenient to keep the focus at a contant distance above the cover
 
 To use a different offset, turn off the autofocus, focus manually on a different position and turn it on again. For convenience, the toolbar also contains "HW-AF ON" and "OFF" buttons. The button "AutoFocus NOW" has no function (it works only with devices from other manufacturers).
  
-### B. Stage positions with multiple offsets
+#### B. Stage positions with multiple offsets
 
 The following procedure describes the setup of a multiposition time series with multiple offsets (i.e., positions may have different z-coordinates above the cover glass).
 1. In the Time lapse tab, check "Time-lapse series" and the "Autofocus" option. Click the "Config" button right of the "Autofocus" option
@@ -195,6 +179,24 @@ The following procedure describes the setup of a multiposition time series with
 
 ![image](uploads/fba83b5bf31852d6c3a8e237896767c0/image.png)
 
+## Shutdown
+- On the microscope, press the ESC button to bring the objective in a safe position.
+- If using CO2 control:
+   - Turn off the CO2 flow on the CO2 controller.
+   - Power off the controller.
+   - Close the main valve of the CO2 tank.
+- Remove your sample.
+- Clean all used immersion objectives with lens cleaning paper.
+
+![Cleaning](uploads/a8aa76b103e7e28022be55e048ecc5b0/Cleaning.jpg){width=30%}
+
+- Exit VisiView.
+- When leaving the system for the next user: sign out from Windows.
+- Full shutdown:
+   - Shut down the computer.
+   - Swith off all laser keys (4).
+   - Power off the power strips in reverse order (3, 2, 1).
+
 # Technical Specifications
 
 ## Objectives