Update Live Spinning Disk authored by Lukas Velas's avatar Lukas Velas
...@@ -22,35 +22,28 @@ ...@@ -22,35 +22,28 @@
- Switch on the lasers you need via the keys (4). 405, 488, 561 and 640 nm are available. - Switch on the lasers you need via the keys (4). 405, 488, 561 and 640 nm are available.
- Turn on the computer (5). - Turn on the computer (5).
![grafik](uploads/42a0189bb49133253436129dae9e7c00/grafik.png) ![grafik](uploads/2af1f6fdc5e072d7ad74e202e18a72de/grafik.png)
### 2. Unit and Camera selection ### 2. Camera selection
- Two spinning disc units are available: W1 and X1 - The W1 unit has the option for sCMOS or EM-CCD camera
- X1 unit has smaller field of view, smaller pinhole spacing (for thinner samples) and higher illumination power density
- W1 unit has larger field of view, larger pinhole spacing (for thicker samples) and lower illumination power density
![grafik](uploads/5cce67415472f3b97d85de58af9dabc1/grafik.png)
- Both units have the option for sCMOS or EM-CCD camera
- sCMOS camera has smaller pixels (higer image resolution) and is suitable for brighter samples - sCMOS camera has smaller pixels (higer image resolution) and is suitable for brighter samples
- EM-CCD camera has larger pixels (lower image resolution) and is suitable for dim samples - EM-CCD camera has larger pixels (lower image resolution) and is suitable for dim samples
- In case of using X1 unit: For the EM-CCD, pull the handle on the spinning disk unit up and put the small plastic box underneath to fix it (see image right). For the sCMOS, push it down. Hold it with your hand while pushing (see image left).
![image](uploads/f09490e159576425b22f3e89732eb30b/image.png)
### 3. Computer ### 3. Computer
- Windows login: pick the right user account (Imaging, FRAP or Nanodissection). Nanodissection is password protected, the others are not. - Windows login: pick the "Imaging" user account. (Also for FRAP)
- Launch VisiView. If you have not turned on all lasers, an info message will list inactive lasers being emulated (Obis, Obis-2, and/or Toptica for the 405, 488, and/or 640 nm, respectively). Acknowledge by clicking OK. Likewise, acknowledge Hard drive not found warnings (the startup script will reset the path for saving files to the standard hard drive D:). - Launch VisiView. If you have not turned on all lasers, an info message will list inactive lasers being emulated (Obis, Obis-2, and/or Toptica for the 405, 488, and/or 640 nm, respectively). Acknowledge by clicking OK. Likewise, acknowledge Hard drive not found warnings (the startup script will reset the path for saving files to the standard hard drive D:).
- Startup dialog: - Startup dialog:
- Camera & Spinning Disk: enter the selected camera & SD (W1 or X1, sCMOS or EM-CCD) - Camera: enter the selected camera (sCMOS or EM-CCD)
- Objective: choose the one you wish to use from the drop-down list - Objective: choose the one you wish to use from the drop-down list
- Objective to load position:
- Yes (recommended) moves the objective to the lowest (safe) position - Yes (recommended) moves the objective to the lowest (safe) position
- No (are you sure?) should only be chosen if you had to restart VisiView during a session - No (are you sure?) should only be chosen if you had to restart VisiView during a session and you dont need to refocus
- Keep settings/positions: - Keep settings/positions:
- No (recommended) clears any previous stage positions and illumination settings - No (recommended) clears any previous stage positions and illumination settings
- Yes should only be chosen if you had to restart VisiView during a session - Yes should only be chosen if you had to restart VisiView during a session
![grafik](uploads/af955893152110fb2c8606ad6154dd71/grafik.png) ![grafik](uploads/14e5c439fc2b39f18d09b60acc98c977/grafik.png)
### 4. Focus and escape position ### 4. Focus and escape position
- After running the startup dialog, the objective should be in the lowest (safe) position. - After running the startup dialog, the objective should be in the lowest (safe) position.
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