Instruments/Live-Spinning-Disk.md

@@ -149,10 +149,10 @@ Also, there are many useful videos from the company: https://www.youtube.com/cha
 
 ### 1. Choosing the right illumination
 
-In the 'illumination' dropdown menu, you can choose the illumination that matches your hardware settings - meaning the selected SD & camera (W1 or X1, sCMOS or EM-CCD). Choose the laser line you need to excite your fluorophore (DAPI, GFP, mCherry & Cy5 - meaning 405, 488, 561 and 647 excitation lasers). The 'Quadband' illuminations have a filter that works for all wavelengths (so your recordings will be faster in case of multicolor imaging), however you will potentially experience more crosstalk, which you can check under https://www.fpbase.org/spectra/. If you are not sure, ask the facility for their help in figuring out whether you can use the Quadband. For each illumination (in case of multi-color imaging) go to 'Show live' adapt the following parameters to achieve the desired image quality (in that order):
+In the 'illumination' dropdown menu, you can choose the illumination that matches your experiment. Choose the laser line you need to excite your fluorophore (DAPI, GFP, mCherry & Cy5 - meaning 405, 488, 561 and 647 excitation lasers). The 'Quadband' illuminations have a filter that works for all wavelengths (so your recordings will be faster in case of multicolor imaging), however you will potentially experience more crosstalk, which you can check under https://www.fpbase.org/spectra/. If you are not sure, ask the facility for their help in figuring out whether you can use the Quadband. For each illumination (in case of multi-color imaging) go to 'Show live' adapt the following parameters to achieve the desired image quality (in that order):
 - the EMCCD Gain (only available for the EMCCD, a good starting value is 300), which only increases your signal on the detection side (no increase in photobleaching)
 - the Exposure (if you have a mobile sample, you might save time by reducing the illumination time)
-- the Laser power (be aware that higher laser powers and illumination times can result in irreversible photobleaching, which impairs e.g. brightness analysis). Do not use more than 10% of the laser power! If you are getting close to 10%, let the facility know and they will realign the lasers
+- the Laser power (be aware that higher laser powers and illumination times can result in irreversible photobleaching, which impairs e.g. brightness analysis).
 
 When acquiring the brightfield channel, you only have to adapt the intensity of the Dialamp.