Instruments/Zeiss-Elyra-7.md

@@ -94,6 +94,11 @@ Example of a Track1 with 488 and 561 nm lasers activated, using DL QUAD 1 SBS LP
 Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beamsplitter
 ![image](uploads/b6b2a89d5421a271fe67ee787a838a7a/image.png){width=412 height=735}
 
+- In the Channels window activate the needed Tracks and Cameras and assign respective colors
+![image](uploads/472840acf0b30c8fdab9bfedef5e94e9/image.png){width=414 height=187}
+- Click on Live to start imaging, adjust laser powers and exposure times for the different tracks and cameras
+- You should aim to achieve at least a Signal to Background ratio of 5 in your image (The structures of interest should be at least 5 times brighter than background e.g. Signal 500 / Background 100)
+
 [Zeiss_Elyra_Setting_up_Lattice_SIM.pdf](uploads/8a812e218450d11bab8caa13642c6510/Zeiss_Elyra_Setting_up_Lattice_SIM.pdf)
 
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