Instruments/Zeiss-Elyra-7.md

@@ -97,9 +97,15 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
 - In the Channels window activate the needed Tracks and Cameras and assign respective colors
 
 ![image](uploads/472840acf0b30c8fdab9bfedef5e94e9/image.png){width=414 height=187}
-- Click on Live to start imaging, adjust laser powers and exposure times for the different tracks and cameras
+- Click on Live to start imaging, refocus on your sample, adjust laser powers and exposure times for the different tracks and cameras
 - You should aim to achieve at least a Signal to Background ratio of 5 in your image (The structures of interest should be at least 5 times brighter than background e.g. Signal 500 / Background 100)
 
+
+- Next, move to the Acquisition Mode window; Select as small ROI as needed for your sample (reduces file size)
+- In Noise Filter click "On - TIRF or WF"
+- Choose Number Phases: 13 phases provide max resolution for optimal SIM, 9 phases provide less resolution but are faster (therefore also less photobleaching)
+- ![image](uploads/b170724c07d05e57502f851747d9268e/image.png){width=410 height=478}
+
 ![image](uploads/e3cb7873d8cbed4ccb2191e2a0c1d9dc/image.png){width=510 height=289}
 
 [Zeiss_Elyra_Setting_up_Lattice_SIM.pdf](uploads/8a812e218450d11bab8caa13642c6510/Zeiss_Elyra_Setting_up_Lattice_SIM.pdf)