Update Zeiss Elyra 7 authored by Clara Bodner's avatar Clara Bodner
...@@ -20,9 +20,22 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet ...@@ -20,9 +20,22 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet
2. Image Setup for camera 2 (TV2) calibration: lattice SIM, LBF 405/488/561/642, LP560, use the biggest (42µm) grid 2. Image Setup for camera 2 (TV2) calibration: lattice SIM, LBF 405/488/561/642, LP560, use the biggest (42µm) grid
3. Focus on the lattice pattern and take a ‘Snap’ 3. Focus on the lattice pattern and take a ‘Snap’
4. Go to ‘Graphics’ and draw an arrow/line across the pattern 4. Go to ‘Graphics’ and draw an arrow/line across the pattern
![Graphics](uploads/a5c85c9914eee4c16a20224af2c69ba5/Graphics.jpg)
![Grid](uploads/a1e938d78726d18e1c3285c271ab996e/Grid.jpg)
5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation 5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
![Grid_left](uploads/a87bec7600fdf31fe9b0065a2f23a0f4/Grid_left.jpg)![Grid_right](uploads/662b603921d038da3c9954f49d771493/Grid_right.jpg)![Dimensions](uploads/b3c918277fc692ab3a6e923208c0dc5a/Dimensions.jpg)
6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle 6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
![Graphics_measure](uploads/8de9fc67bee34262465186b8022c2c1f/Graphics_measure.jpg)![Grid_angle](uploads/fbea05bb3dba5378465ed6d94f36d71a/Grid_angle.jpg)
7. Go to ‘Maintain’, and drag out the ‘Dual Camera Adapter Alignment’ tool 7. Go to ‘Maintain’, and drag out the ‘Dual Camera Adapter Alignment’ tool
![Maintain](uploads/4cd5fe821496c21336794064bfb57643/Maintain.jpg)
8. Tick 'Calibration pattern' 8. Tick 'Calibration pattern'
8. Change the ‘Rot [°]’ value. Take another snap of the marker sample and measure the angle again, change the ‘Rot [°]’ value again. Repeat until you measure a value lower than 0.05°. Store current position in the ‘Dual Camera Alignment’ tool 8. Change the ‘Rot [°]’ value. Take another snap of the marker sample and measure the angle again, change the ‘Rot [°]’ value again. Repeat until you measure a value lower than 0.05°. Store current position in the ‘Dual Camera Alignment’ tool
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