Update Zeiss Elyra 7 authored by Clara Bodner's avatar Clara Bodner
......@@ -20,9 +20,22 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet
2. Image Setup for camera 2 (TV2) calibration: lattice SIM, LBF 405/488/561/642, LP560, use the biggest (42µm) grid
3. Focus on the lattice pattern and take a ‘Snap’
4. Go to ‘Graphics’ and draw an arrow/line across the pattern
![Graphics](uploads/a5c85c9914eee4c16a20224af2c69ba5/Graphics.jpg)
![Grid](uploads/a1e938d78726d18e1c3285c271ab996e/Grid.jpg)
5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
![Grid_left](uploads/a87bec7600fdf31fe9b0065a2f23a0f4/Grid_left.jpg)![Grid_right](uploads/662b603921d038da3c9954f49d771493/Grid_right.jpg)![Dimensions](uploads/b3c918277fc692ab3a6e923208c0dc5a/Dimensions.jpg)
6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
![Graphics_measure](uploads/8de9fc67bee34262465186b8022c2c1f/Graphics_measure.jpg)![Grid_angle](uploads/fbea05bb3dba5378465ed6d94f36d71a/Grid_angle.jpg)
7. Go to ‘Maintain’, and drag out the ‘Dual Camera Adapter Alignment’ tool
![Maintain](uploads/4cd5fe821496c21336794064bfb57643/Maintain.jpg)
8. Tick 'Calibration pattern'
8. Change the ‘Rot [°]’ value. Take another snap of the marker sample and measure the angle again, change the ‘Rot [°]’ value again. Repeat until you measure a value lower than 0.05°. Store current position in the ‘Dual Camera Alignment’ tool
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