Update Zeiss Elyra 7 authored by Nicholas Wedige's avatar Nicholas Wedige
...@@ -67,17 +67,44 @@ Always make sure that the system is back at 27°C and the CO<sub>2</sub> is off ...@@ -67,17 +67,44 @@ Always make sure that the system is back at 27°C and the CO<sub>2</sub> is off
</details> </details>
## Environmental Control ## Acquisition Setup
[Zeiss_Elyra_Environmental_controls_01.pdf](uploads/9e4a1088f421fd1742a2dd0076f3a89a/Zeiss_Elyra_Environmental_controls_01.pdf) <details>
<summary>
## Setting up Lattice SIM ### `Setting up Lattice SIM`
</summary>
[Zeiss_Elyra_Setting_up_Lattice_SIM.pdf](uploads/8a812e218450d11bab8caa13642c6510/Zeiss_Elyra_Setting_up_Lattice_SIM.pdf) [Zeiss_Elyra_Setting_up_Lattice_SIM.pdf](uploads/8a812e218450d11bab8caa13642c6510/Zeiss_Elyra_Setting_up_Lattice_SIM.pdf)
## Setting up TIRF </details>
<details>
<summary>
### `Setting up TIRF`
</summary>
[Zeiss_Elyra_Setting_up_TIRF_imaging.pdf](uploads/d5abb8b971c53cbf7a21b318be59d1c3/Zeiss_Elyra_Setting_up_TIRF_imaging.pdf) [Zeiss_Elyra_Setting_up_TIRF_imaging.pdf](uploads/d5abb8b971c53cbf7a21b318be59d1c3/Zeiss_Elyra_Setting_up_TIRF_imaging.pdf)
<details>
<summary>
### `Environmental Control`
</summary>
</details>
[Zeiss_Elyra_Environmental_controls_01.pdf](uploads/9e4a1088f421fd1742a2dd0076f3a89a/Zeiss_Elyra_Environmental_controls_01.pdf)
</details>
## Dual-Camera Alignment & Colocalization Analysis ## Dual-Camera Alignment & Colocalization Analysis
Camera Alignment has to be checked/done before **each experiment** using both cameras and for **each used filter** in the camera link and for **each used objective** individually. It has to be done again if the camera was pushed by accident, or if the cover of the camera link has been opened. Camera Alignment has to be checked/done before **each experiment** using both cameras and for **each used filter** in the camera link and for **each used objective** individually. It has to be done again if the camera was pushed by accident, or if the cover of the camera link has been opened.
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