Instruments/Zeiss-Elyra-7.md

@@ -130,12 +130,14 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
 
 - In the Acquisition Tab, Imaging Setup window, add SMLM track bz clicking +SMLM from the drop down window.
 - Set up your imaging path: 
-- 1) Choose laser, 
-- 2) TIRF for the field of view lens,
-- 3) Illumination mode (EPI/HILO/TIRF),
-- 4) don't change the Collimator,
-- 5) FS LBF 405/488/561/642 beam splitter, 
-- 6) use 3D SMLM slider onlz for 3D SMLM with double helix PSF, 
+1) Choose laser, 
+2) choose TIRF for the field of view lens,
+3) set illumination mode to EPI/HILO/TIRF,
+4) don't change the Collimator,
+5) choose FS LBF 405/488/561/642 beam splitter, 
+6) use 3D SMLM slider only for 3D SMLM with double helix PSF,
+7) pick 100x 1.46NA or 63x 1.46NA objective
+8) on the emission side, choose 1x Tube lens and a suitable DuoLink beam splitter
 
 [Zeiss_Elyra_Setting_up_TIRF_imaging.pdf](uploads/d5abb8b971c53cbf7a21b318be59d1c3/Zeiss_Elyra_Setting_up_TIRF_imaging.pdf)