... | ... | @@ -82,7 +82,13 @@ Always make sure that the system is back at 27°C and the CO<sub>2</sub> is off |
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Camera Alignment has to be checked/done before **each experiment** using both cameras and for **each used filter** in the camera link and for **each used objective** individually. It has to be done again if the camera was pushed by accident, or if the cover of the camera link has been opened.
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### Camera Rotation
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<details>
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<summary>
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### `Camera Rotation`
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</summary>
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Use the yellow marker pen slide with a coverslip (on the desk next to the lens cleaning paper) and the slide with beads (multispec low, they are stored in the fridge close to the Elyra).
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... | ... | @@ -114,7 +120,17 @@ Use the yellow marker pen slide with a coverslip (on the desk next to the lens c |
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12. Untick 'Calibration pattern'
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### Camera xyz-Alignment
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</details>
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<details>
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<summary>
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### `Camera xyz-Alignment`
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</summary>
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1. Use the 63x 1.4 (SIM) or 1.46 (TIRF) objective and the bead slide and focus on beads
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2. Use the same imaging method (LASER WF, SIM, Apotome etc.) and the same filter settings as for your experiment!
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... | ... | @@ -143,7 +159,15 @@ Use the yellow marker pen slide with a coverslip (on the desk next to the lens c |
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### Channel Alignment
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</details>
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<details>
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<summary>
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### `Channel Alignment`
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</summary>
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Channel Alignment has to be done before **each colocalization experiment** using multiple color channels and for **each used filter** in the camera link, for **each used objective** and for **each configuration of different cameras/tracks** individually. A bead sample has to be prepared by the user beforehand, the exact same way (same mounting etc.) as the actual sample. If your lab does not have beads, contact the facility staff and they will show you which ones to purchase (Tetraspeck 100nm beads, they are stored in the fridge's door close to the Elyra).
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... | ... | @@ -157,6 +181,10 @@ Channel Alignment has to be done before **each colocalization experiment** using |
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5. Save the results in order to correct your images later on
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6. You can apply your correction together with the SIM processing
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</details>
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# Data Processing
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## Data Handling and Transfer
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