Update Zeiss Elyra 7 authored by Lukas Velas's avatar Lukas Velas
......@@ -100,13 +100,15 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
- Click on Live to start imaging, refocus on your sample, adjust laser powers and exposure times for the different tracks and cameras
- You should aim to achieve at least a Signal to Background ratio of 5 in your image (The structures of interest should be at least 5 times brighter than background e.g. Signal 500 / Background 100)
![image](uploads/e3cb7873d8cbed4ccb2191e2a0c1d9dc/image.png){width=510 height=289}
- Next, move to the Acquisition Mode window; Select as small ROI as needed for your sample (reduces file size)
- In Noise Filter click "On - TIRF or WF"
- Choose Number Phases: 13 phases provide max resolution for optimal SIM, 9 phases provide less resolution but are faster (therefore also less photobleaching)
- ![image](uploads/b170724c07d05e57502f851747d9268e/image.png){width=410 height=478}
![image](uploads/e3cb7873d8cbed4ccb2191e2a0c1d9dc/image.png){width=510 height=289}
![image](uploads/b170724c07d05e57502f851747d9268e/image.png){width=410 height=478}
[Zeiss_Elyra_Setting_up_Lattice_SIM.pdf](uploads/8a812e218450d11bab8caa13642c6510/Zeiss_Elyra_Setting_up_Lattice_SIM.pdf)
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