Update Zeiss Elyra 7 authored by Lukas Velas's avatar Lukas Velas
...@@ -79,7 +79,7 @@ Always make sure that the system is back at 28.5°C and the CO<sub>2</sub> is of ...@@ -79,7 +79,7 @@ Always make sure that the system is back at 28.5°C and the CO<sub>2</sub> is of
</summary> </summary>
- Move to Acquisition Tab - Move to the Acquisition Tab
- In the Imaging Setup window click on +Lattice SIM: This will create a Track1, if you need more tracks click additionally on +Lattice SIM - In the Imaging Setup window click on +Lattice SIM: This will create a Track1, if you need more tracks click additionally on +Lattice SIM
- Activate respective lasers (405, 488, 561 and 642 nm are available) - Activate respective lasers (405, 488, 561 and 642 nm are available)
- In the SIM Grating changer 'Optimal Grating' should be checked - In the SIM Grating changer 'Optimal Grating' should be checked
...@@ -124,7 +124,7 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam ...@@ -124,7 +124,7 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
<summary> <summary>
### `Setting up TIRF` ### `Setting up TIRF (old)`
</summary> </summary>
...@@ -144,7 +144,7 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam ...@@ -144,7 +144,7 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
</details> </details>
## Dual-Camera Alignment & Colocalization Analysis ## Dual-Camera Alignment & Colocalization Analysis (old)
Camera Alignment has to be checked/done before **each experiment** using both cameras and for **each used filter** in the camera link and for **each used objective** individually. It has to be done again if the camera was pushed by accident, or if the cover of the camera link has been opened. Camera Alignment has to be checked/done before **each experiment** using both cameras and for **each used filter** in the camera link and for **each used objective** individually. It has to be done again if the camera was pushed by accident, or if the cover of the camera link has been opened.
...@@ -152,7 +152,7 @@ Camera Alignment has to be checked/done before **each experiment** using both ca ...@@ -152,7 +152,7 @@ Camera Alignment has to be checked/done before **each experiment** using both ca
<summary> <summary>
### `Camera Rotation` ### `Camera Rotation (old)`
</summary> </summary>
...@@ -189,7 +189,7 @@ Use the yellow marker pen slide with a coverslip (on the desk next to the lens c ...@@ -189,7 +189,7 @@ Use the yellow marker pen slide with a coverslip (on the desk next to the lens c
<summary> <summary>
### `Camera xyz-Alignment` ### `Camera xyz-Alignment (old)`
</summary> </summary>
...@@ -219,7 +219,7 @@ Use the yellow marker pen slide with a coverslip (on the desk next to the lens c ...@@ -219,7 +219,7 @@ Use the yellow marker pen slide with a coverslip (on the desk next to the lens c
<summary> <summary>
### `Channel Alignment` ### `Channel Alignment (old)`
</summary> </summary>
...@@ -274,7 +274,7 @@ Channel Alignment has to be done before **each colocalization experiment** using ...@@ -274,7 +274,7 @@ Channel Alignment has to be done before **each colocalization experiment** using
<summary> <summary>
### `Data Handling and Transfer` ### `Data Handling and Transfer (old)`
</summary> </summary>
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