Update Zeiss Elyra 7 authored by Lukas Velas's avatar Lukas Velas
...@@ -240,15 +240,29 @@ Channel Alignment has to be done before **each colocalization experiment** using ...@@ -240,15 +240,29 @@ Channel Alignment has to be done before **each colocalization experiment** using
## Data Processing ## Data Processing
hi1 - Move to the Processing Tab
- In the Method drop down search for SIM Processing
<details> ![image](uploads/580e98c17829d0eb242524cd91b2ebee/image.png){width=414 height=431}
hi2 - Select the image you want to process in the Input window
<summary> ![image](uploads/a1733f977571c0b52f0c1717a7b38e5a/image.png){width=413 height=326}
- In the Parameters window, put in the desired processing parameters
- The following parameters are recommended as a good start with SIM processing (Linear Deconvolution)
![image](uploads/b3c19fc1f7bb4a685d25e2c0f4f7eb23/image.png){width=413 height=690}
- The following parameters are recommended as a good start with SIM2 processing (Iterative Deconvolution)
hi3 ![image](uploads/319f4ae3ea65be85f5d2b56f4672c5ad/image.png){width=408 height=837}
- You can further adjust the parameters if you think that the "starting parameters" gave you a reasonable result without artefacts
<details>
<summary>
### `Data Handling and Transfer` ### `Data Handling and Transfer`
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