4. Start the 'Zen black' Software and press 'Start up System'
4. Start the 'ZEISS ZEN' Software and press 'ZEN - Full ZEN Functionality'
5. The temperature inside the incubator will be by default at 27°C to minimize drift. Use the oil for 30°C. Immersion oil bottles for 30°C and 37°C are stored in the incubator at the bottom right.
5. The temperature inside the incubator will be by default at 28.5°C to minimize drift. For the oil immersion objectives use the oil for 30°C. Immersion oil bottles for 30°C and 37°C are stored in the incubator at the bottom right.
6. Put on your sample and focus on it using the 'Locate' tab in the Zen Software (press 'Fluorescence' or 'Transmitted').
6. Put on your sample and focus on it using the 'Locate' tab in the Zen Software (press 'Fluorescence' or 'Bright Field', when done 'OFF'). The fluorescence lamp is very bright, for light sensitive samples skip Locate and focus in the Aquisition Tab.
7. With the front window open and the light in the incubator on (switch to the left, on the microscope table) you can safely navigate larger distances when using the joystick (which has an inverse logic).
7. With the front window open and the light in the incubator on (switch to the left, on the microscope table) you can safely navigate larger distances when using the joystick (which has an inverse logic).
For the front flap you will need to open (to move flap up) and tighten (to keep the flap in place) the little red screw. Please handle this window with care!
For the front flap you will need to open (to move flap up) and tighten (to keep the flap in place) the little red screw. Please handle this window with care!
