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Update Zeiss Elyra 7 authored by Clara Bodner's avatar Clara Bodner
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Instruments/Zeiss-Elyra-7.md
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...@@ -24,13 +24,13 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet ...@@ -24,13 +24,13 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet
3. Focus on the lattice pattern and take a ‘Snap’ 3. Focus on the lattice pattern and take a ‘Snap’
4. Go to ‘Graphics’ and draw an arrow/line across the pattern 4. Go to ‘Graphics’ and draw an arrow/line across the pattern
![Graphics](uploads/a5c85c9914eee4c16a20224af2c69ba5/Graphics.jpg){width=30%} ![Graphics](uploads/a5c85c9914eee4c16a20224af2c69ba5/Graphics.jpg){width=50%}
![Grid](uploads/a1e938d78726d18e1c3285c271ab996e/Grid.jpg){width=100%} ![Grid](uploads/a1e938d78726d18e1c3285c271ab996e/Grid.jpg){width=100%}
5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation 5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
![Grid_left](uploads/a87bec7600fdf31fe9b0065a2f23a0f4/Grid_left.jpg){width=30%}![Grid_right](uploads/662b603921d038da3c9954f49d771493/Grid_right.jpg){width=30%} ![Grid_left](uploads/a87bec7600fdf31fe9b0065a2f23a0f4/Grid_left.jpg){width=30%} ![Grid_right](uploads/662b603921d038da3c9954f49d771493/Grid_right.jpg){width=28%}
![Dimensions](uploads/b3c918277fc692ab3a6e923208c0dc5a/Dimensions.jpg){width=30%} ![Dimensions](uploads/b3c918277fc692ab3a6e923208c0dc5a/Dimensions.jpg){width=50%}
6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle 6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
...@@ -68,7 +68,8 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet ...@@ -68,7 +68,8 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet
15. In Dual Camera Adapter Alignment tool click XY for automatic alignment, if good overlap of channels is achieved, store current position, if not – move to stored position and in 'Continuous' mode change X and Y in the Dual Camera Adapter Alignment tool until overlap is achieved. Especially the beads in the center of the field of view should nicely overlap. Use the 'Profile > Display > Marker > Difference' tool to check the overlap. 15. In Dual Camera Adapter Alignment tool click XY for automatic alignment, if good overlap of channels is achieved, store current position, if not – move to stored position and in 'Continuous' mode change X and Y in the Dual Camera Adapter Alignment tool until overlap is achieved. Especially the beads in the center of the field of view should nicely overlap. Use the 'Profile > Display > Marker > Difference' tool to check the overlap.
![Dual_camera_adapter_alignment_xy](uploads/1224a127963f3b439bf0547942ef9375/Dual_camera_adapter_alignment_xy.jpg){width=30%}![beads_xy](uploads/78d4f493776d040aacaddeff9ff0b495/beads_xy.jpg){width=30%} ![Dual_camera_adapter_alignment_xy](uploads/1224a127963f3b439bf0547942ef9375/Dual_camera_adapter_alignment_xy.jpg){width=30%}
![beads_xy](uploads/78d4f493776d040aacaddeff9ff0b495/beads_xy.jpg){width=30%}
16. Tick 'Calibration pattern' and check XY Overlap. Store current position! 16. Tick 'Calibration pattern' and check XY Overlap. Store current position!
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