| ... | @@ -24,13 +24,13 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet |
... | @@ -24,13 +24,13 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet |
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3. Focus on the lattice pattern and take a ‘Snap’
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3. Focus on the lattice pattern and take a ‘Snap’
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4. Go to ‘Graphics’ and draw an arrow/line across the pattern
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4. Go to ‘Graphics’ and draw an arrow/line across the pattern
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{width=30%}
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{width=50%}
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{width=100%}
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{width=100%}
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5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
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5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
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{width=30%}{width=30%}
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{width=30%} {width=28%}
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{width=30%}
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{width=50%}
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6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
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6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
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| ... | @@ -68,7 +68,8 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet |
... | @@ -68,7 +68,8 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet |
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15. In Dual Camera Adapter Alignment tool click XY for automatic alignment, if good overlap of channels is achieved, store current position, if not – move to stored position and in 'Continuous' mode change X and Y in the Dual Camera Adapter Alignment tool until overlap is achieved. Especially the beads in the center of the field of view should nicely overlap. Use the 'Profile > Display > Marker > Difference' tool to check the overlap.
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15. In Dual Camera Adapter Alignment tool click XY for automatic alignment, if good overlap of channels is achieved, store current position, if not – move to stored position and in 'Continuous' mode change X and Y in the Dual Camera Adapter Alignment tool until overlap is achieved. Especially the beads in the center of the field of view should nicely overlap. Use the 'Profile > Display > Marker > Difference' tool to check the overlap.
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{width=30%}{width=30%}
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{width=30%}
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{width=30%}
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16. Tick 'Calibration pattern' and check XY Overlap. Store current position!
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16. Tick 'Calibration pattern' and check XY Overlap. Store current position!
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| ... | | ... | |
