Update Zeiss Elyra 7 authored by Clara Bodner's avatar Clara Bodner
......@@ -24,13 +24,13 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet
3. Focus on the lattice pattern and take a ‘Snap’
4. Go to ‘Graphics’ and draw an arrow/line across the pattern
![Graphics](uploads/a5c85c9914eee4c16a20224af2c69ba5/Graphics.jpg){width=30%}
![Graphics](uploads/a5c85c9914eee4c16a20224af2c69ba5/Graphics.jpg){width=50%}
![Grid](uploads/a1e938d78726d18e1c3285c271ab996e/Grid.jpg){width=100%}
5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
![Grid_left](uploads/a87bec7600fdf31fe9b0065a2f23a0f4/Grid_left.jpg){width=30%}![Grid_right](uploads/662b603921d038da3c9954f49d771493/Grid_right.jpg){width=30%}
![Dimensions](uploads/b3c918277fc692ab3a6e923208c0dc5a/Dimensions.jpg){width=30%}
![Grid_left](uploads/a87bec7600fdf31fe9b0065a2f23a0f4/Grid_left.jpg){width=30%} ![Grid_right](uploads/662b603921d038da3c9954f49d771493/Grid_right.jpg){width=28%}
![Dimensions](uploads/b3c918277fc692ab3a6e923208c0dc5a/Dimensions.jpg){width=50%}
6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
......@@ -68,7 +68,8 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet
15. In Dual Camera Adapter Alignment tool click XY for automatic alignment, if good overlap of channels is achieved, store current position, if not – move to stored position and in 'Continuous' mode change X and Y in the Dual Camera Adapter Alignment tool until overlap is achieved. Especially the beads in the center of the field of view should nicely overlap. Use the 'Profile > Display > Marker > Difference' tool to check the overlap.
![Dual_camera_adapter_alignment_xy](uploads/1224a127963f3b439bf0547942ef9375/Dual_camera_adapter_alignment_xy.jpg){width=30%}![beads_xy](uploads/78d4f493776d040aacaddeff9ff0b495/beads_xy.jpg){width=30%}
![Dual_camera_adapter_alignment_xy](uploads/1224a127963f3b439bf0547942ef9375/Dual_camera_adapter_alignment_xy.jpg){width=30%}
![beads_xy](uploads/78d4f493776d040aacaddeff9ff0b495/beads_xy.jpg){width=30%}
16. Tick 'Calibration pattern' and check XY Overlap. Store current position!
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