Instruments/Zeiss-Elyra-7.md

@@ -129,6 +129,13 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
 </summary>
 
 - In the Acquisition Tab, Imaging Setup window, add SMLM track bz clicking +SMLM from the drop down window.
+- Set up your imaging path: 
+- 1) Choose laser, 
+- 2) TIRF for the field of view lens,
+- 3) Illumination mode (EPI/HILO/TIRF),
+- 4) don't change the Collimator,
+- 5) FS LBF 405/488/561/642 beam splitter, 
+- 6) use 3D SMLM slider onlz for 3D SMLM with double helix PSF, 
 
 [Zeiss_Elyra_Setting_up_TIRF_imaging.pdf](uploads/d5abb8b971c53cbf7a21b318be59d1c3/Zeiss_Elyra_Setting_up_TIRF_imaging.pdf)