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8. All doors on the incubator need to be properly closed before you can start imaging in the Acquisition tab as otherwise the interlock is not released and the lasers will not be active. You can check for this: if one of the LEDs on the main controller is blinking, one of the laser interlocks has not been recognized. Unfortunately, there is no feedback which interlock is affected, so you need to check them all and potentially, open and close all doors again or even lift the condenser and put it back down.
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{width=49%} {width=49%}
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{width=54%} {width=45%}
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## Environmental Control
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[Zeiss_Elyra_Environmental_controls_01.pdf](uploads/9e4a1088f421fd1742a2dd0076f3a89a/Zeiss_Elyra_Environmental_controls_01.pdf)
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