Update Zeiss Elyra 7 authored by Lukas Velas's avatar Lukas Velas
......@@ -191,6 +191,17 @@ Channel Alignment consists of three steps:
2. Finding the coordinate transformation between the two channels. This is a post processing step and can be done after your measurement.
3. Applying the coordinate transformation to your recorded images. This is also a post processing step applied during your image analysis.
Procedure:
- Mount TetraSpeck multicolor beads. Use the same objective as for your sample. Look for a spot with many evenly distributed beads.
![image](uploads/e09fea2951771c34e37710707610f7a6/image.png){width=1065 height=1065}
- Record a 5μm large Z stack around the beads using the same Tracks as for imaging your sample.
![image](uploads/31e5987436d0e02d43ac8f30967c66c5/image.png){width=408 height=405}
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