Instruments/Zeiss-Elyra-7.md

@@ -141,6 +141,9 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
 
 ![image](uploads/e6613a1f6f63b4918c5121b4a95f0567/image.png){width=339 height=600}
 
+- In the Channels window, adjust the laser power, choose the ND Filter and choose exposure Time for the Camera
+- It is not recommended to further change TIRF Angle and Collimator settings without dedicated training from the facility staff
+
 [Zeiss_Elyra_Setting_up_TIRF_imaging.pdf](uploads/d5abb8b971c53cbf7a21b318be59d1c3/Zeiss_Elyra_Setting_up_TIRF_imaging.pdf)
 
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