@@ -79,7 +79,17 @@ Always make sure that the system is back at 28.5°C and the CO<sub>2</sub> is of
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@@ -79,7 +79,17 @@ Always make sure that the system is back at 28.5°C and the CO<sub>2</sub> is of
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hi
- Move to Acquisition Tab
- In the Imaging Setup window click on +Lattice SIM> This will Create a Track1, if you need more tracks click additionally on +Lattice SIM
- Activate respective lasers (405, 488, 561 and 642 nm are available)
- In the SIM Grating changer 'Optimal Grating' should be checked
- Select FS LBF 405/488/561/642 beam splitter
- Choose 1.6x tube lens
- Use 100% R sideport turret
- Choose the correct beamsplitter for your application in the DuoLink (Either Longpass 560nm, or a combination of Bandpass 490-560nm with Longpass 640 nm)
Example of a track with 488 and 561 nm lasers activated, using DL QUAD 1 SBS LP 560 beamsplitter (LP 560 stands for Long Pass 560 nm)
