Update Zeiss Elyra 7 authored by Lukas Velas's avatar Lukas Velas
...@@ -95,6 +95,7 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam ...@@ -95,6 +95,7 @@ Example of a Track2 with 405 nm laser activated, using DL QUAD 1 SBS LP 560 beam
![image](uploads/b6b2a89d5421a271fe67ee787a838a7a/image.png){width=412 height=735} ![image](uploads/b6b2a89d5421a271fe67ee787a838a7a/image.png){width=412 height=735}
- In the Channels window activate the needed Tracks and Cameras and assign respective colors - In the Channels window activate the needed Tracks and Cameras and assign respective colors
![image](uploads/472840acf0b30c8fdab9bfedef5e94e9/image.png){width=414 height=187} ![image](uploads/472840acf0b30c8fdab9bfedef5e94e9/image.png){width=414 height=187}
- Click on Live to start imaging, adjust laser powers and exposure times for the different tracks and cameras - Click on Live to start imaging, adjust laser powers and exposure times for the different tracks and cameras
- You should aim to achieve at least a Signal to Background ratio of 5 in your image (The structures of interest should be at least 5 times brighter than background e.g. Signal 500 / Background 100) - You should aim to achieve at least a Signal to Background ratio of 5 in your image (The structures of interest should be at least 5 times brighter than background e.g. Signal 500 / Background 100)
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