@@ -174,7 +174,22 @@ Camera Alignment has to be checked/done before **each experiment** using both ca
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@@ -174,7 +174,22 @@ Camera Alignment has to be checked/done before **each experiment** using both ca
- At the end, click "Store Calibration" button
- At the end, click "Store Calibration" button
- Keep in mind that the camera alignment will never be perfectly accurate due to chromatic aberrations, and for accurate colocalization studies, you need to perform "Channel Alignment", as described in the following section.
- Keep in mind that the camera alignment will never be perfectly accurate due to chromatic aberrations, and for accurate colocalization studies, you need to perform "Channel Alignment", as described in the following section.
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### `Channel Alignment`
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Channel Alignment needs to be performed for colocalization studies. Only so you can guarantee that the different color channels are not shifted.
Channel Alignment consists of three steps:
1. Recording multicolor beads with the same settings as your sample. This must be done right before or right after your experiment.
2. Finding the coordinate transformation between the two channels. This is a post processing step and can be done after your measurement.
3. Applying the coordinate transformation to your recorded images. This is also a post processing step applied during your image analysis.
