Instruments/Zeiss-LSM-980.md

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 # Profile
 
 | ![20200504_130609](uploads/f43ce4dfe1659b0e2b813414cacad612/20200504_130609.jpg){width=5000} | Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives. |
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 # Manuals & Guides
 
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 ## Startup & Shutdown
 
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 | ![image](uploads/85fc29131f4a43a3594237567465d1c3/image.png){width=100%} | 1. Turn the Main Switch (1) in ON position <br><br> 2. Turn the Laser key (2) clockwise in vertical position (labeled "Laser") <br><br> 3. Push the Components switch (3) in ON position <br><br> 4. Power up the PC (4). Login into the "user" account and start ZEN <br><br> 5. Press the Load position button on the touch panel of the microscope <br><br> |
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 1. Close ZEN
 2. Press the Load position button on the touch panel
 3. Clean immersion objectives with lens cleaning paper
 
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 #### Leaving the system for the next user
 4. Logout from the Windows session
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 #### Full shutdown (last user)
 4. Shut down the PC (4)
 5. Push the Components switch (3) in OFF position
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 7. Turn the Main Switch (1) in OFF position
 8. Cover the microscope with the dust cover
 
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 ## Environmental Control
 
 - Stagetop incubation only
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 If you want plan to use stage-top incubation on the LSM-980, you need to first open (only one turn needed) the valve on the CO<sub>2</sub> bottle itself, then open the respective valve to the microscope. 
 
 Next, assemble the heating unit with the appropriate insert for your sample carrier. There is one insert for regular slides/Ibidi Dishes **(3)** and one for 35mm dishes **(4)**
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 **H Insert P**: insert for slides/ibidi dishes  
 **H Insert M**: insert for 35mm dishes  
 
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 ![heatingfull3](uploads/58b747dd60df30c0fb774fa2bdd40fe2/heatingfull3.jpg)
 
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 ## Acquisition Setup
 
 The LSM-980 can be used in various modes, the "standard" confocal mode on the one hand and a variety of imaging modalities using the Airyscan detector. Depending on the modalities selected, setting up your acquisition will vary slightly and different aspects need to be considered.  
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 - Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
 - MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
 
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 # Technical Specifications
 
 ## Objectives
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 ## Lasers
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 | Wavelength [nm] | Power [mW] | Type  |
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 | $`\textcolor{purple}{\sf{405}}`$           | 30         | diode |