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</details>
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### Environmental Control
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<details>
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<summary>
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#### `Setting up Incubation`
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</summary>
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<br/>
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- Stagetop incubation only
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- Controller: Zeiss TempModule S, CO<sub>2</sub> Module S
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If you want plan to use stage-top incubation on the LSM-980, you need to first open (only one turn needed) the valve on the CO<sub>2</sub> bottle itself, then open the respective valve to the microscope.
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Next, assemble the heating unit with the appropriate insert for your sample carrier. There is one insert for regular slides/Ibidi Dishes **(3)** and one for 35mm dishes **(4)**
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For assembly, insert the heated sample holder (**3** or **4**) in the holding frame **(1)**.
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Next, click the assembled holding frame with the insert into the stage of the microscope.
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Finally add the heated lid - it should fit exactly over the heated insert. The fully assembled heating unit is shown in **(5)**. The orientation of the insert is principally irrelevant, but the most sensible setup would be with the heating cables leading to the right.
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**Be careful when lowering the condenser, as it could be in a too low position, which would lead to the condenser crashing into the heating lid!**
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In the software, move to the "Incubation" Tab (in the right hand panel in ZEN) and tick the heated lid and one of the inserts (the one you use) and the components will be heated to the selected temperature. Finally tick "CO<sub>2</sub> Small V", assuming you need to supply CO<sub>2</sub>.
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**Inc PM**: lid
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**H Insert P**: insert for slides/ibidi dishes
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**H Insert M**: insert for 35mm dishes
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</details>
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