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Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives.
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Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives.
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<details open><summary> ## Manuals & Guides </summary>
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- [Startup/Shutdown Guide](Instruments/DeltaVision-Ultra/Startup+Shutdown Guide)
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## Manuals & Guides
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### Airyscanning
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- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
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- Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
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- MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
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### Startup & Shutdown Guide
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#### Startup
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1. Turn the Main Switch (1) in ON position
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2. Turn the Laser key (2) clockwise in vertical position (labeled "Laser")
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3. Push the Components switch (3) in ON position
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4. Power up the PC (4). Login into the "user" account and start ZEN
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5. Press the Load position button on the touch panel of the microscope
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{width=75%}
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#### Shutdown
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1. Close ZEN
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2. Press the Load position button on the touch panel
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3. Clean immersion objectives with lens cleaning paper
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##### Leaving the system for the next user
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4. Logout from the Windows session
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##### Full shutdown (last user)
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4. Shut down the PC (4)
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5. Push the Components switch (3) in OFF position
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6. Turn the Laser key (2) counter-clockwise in horizontal position (labeled "OFF")
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7. Turn the Main Switch (1) in OFF position
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8. Cover the microscope with the dust cover
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</details>
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## Manuals & Guides
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## Manuals & Guides
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### Airyscanning
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### Airyscanning
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- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
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- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
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