Instruments/Zeiss-LSM-980.md

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 # Profile
 
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 | ![20200504_130609](uploads/f43ce4dfe1659b0e2b813414cacad612/20200504_130609.jpg){width=5000} | Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives. |
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 # Manuals & Guides
 
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 ## Startup & Shutdown
 
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 ## Acquisition Setup
 
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 The LSM-980 can be used in various modes, the "standard" confocal mode on the one hand and a variety of imaging modalities using the Airyscan detector. Depending on the modalities selected, setting up your acquisition will vary slightly and different aspects need to be considered.  
   
 In both cases the "Smart Setup" option is a good point to start from. 
 
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 ### Experiment Setups
 
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 In the ZEN blue software there are a myriad of imaging modalities which you can utilize to answer your research questions. Here you can find guides for setting up simple Z-stacks, but also tutorials on configuring more intricate, multidimensional experiments. These manuals are updated and expanded on a regular basis, so check regularly, in case there are new functions or insights. 
 
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