Instruments/Zeiss-LSM-980.md

@@ -59,17 +59,18 @@ For assembly, insert the heated sample holder (**3** or **4**) in the holding fr
 
 Next, click the assembled holding frame with the insert into the stage of the microscope.
 
-Finally add the heated lid, it should fit exactly over the heated insert. The fully assembled heating unit is shown in **(5)** 
+Finally add the heated lid - it should fit exactly over the heated insert. The fully assembled heating unit is shown in **(5)** 
 
 
 
-In the software, move to the "Incubation" Tab (in the right hand panel in ZEN) and tick the heated lid and one of the inserts (the one you use) and the components will be heated to the selected temperature. 
+In the software, move to the "Incubation" Tab (in the right hand panel in ZEN) and tick the heated lid and one of the inserts (the one you use) and the components will be heated to the selected temperature. Finally tick "Co2 Small V", assuming you need to supply Co2. 
 
 **Inc PM**: lid  
 **H Insert P**: insert for slides/ibidi dishes  
 **H Insert M**: insert for 35mm dishes  
 
 
+
 ![heatingfull3](uploads/58b747dd60df30c0fb774fa2bdd40fe2/heatingfull3.jpg)