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</details>
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## Environmental Control
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- Stagetop incubation only
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- Controller: Zeiss TempModule S, CO<sub>2</sub> Module S
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## Acquisition Setup
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<details>
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<summary>
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### `Setting up Incubation`
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### `Environmental Control`
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</summary>
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- Stagetop incubation only
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- Controller: Zeiss TempModule S, CO<sub>2</sub> Module S
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If you want plan to use stage-top incubation on the LSM-980, you need to first open (only one turn needed) the valve on the CO<sub>2</sub> bottle itself, then open the respective valve to the microscope.
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Next, assemble the heating unit with the appropriate insert for your sample carrier. There is one insert for regular slides/Ibidi Dishes **(3)** and one for 35mm dishes **(4)**
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</details>
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## Acquisition Setup
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The LSM-980 can be used in various modes, the "standard" confocal mode on the one hand and a variety of imaging modalities using the Airyscan detector. Depending on the modalities selected, setting up your acquisition will vary slightly and different aspects need to be considered.
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In both cases the "Smart Setup" option is a good point to start from.
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