| ... | ... | @@ -3,7 +3,10 @@ |
|
|
|
Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives.
|
|
|
|
|
|
|
|
## Manuals & Guides
|
|
|
|
|
|
|
|
### Airyscanning
|
|
|
|
- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
|
|
|
|
- Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
|
|
|
|
- MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
|
|
|
|
### Startup & Shutdown Guide
|
|
|
|
|
|
|
|
### Sample Preparation
|
| ... | ... | @@ -12,10 +15,7 @@ Installed 2020. Inverse point scanning confocal microscope with up to 6 channels |
|
|
|
|
|
|
|
## Technical Specifications
|
|
|
|
|
|
|
|
### Airyscanning
|
|
|
|
- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
|
|
|
|
- Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
|
|
|
|
- MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
### Objectives
|
| ... | ... | |
