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### STED
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| {width="8cm"} | Upright point scanning microscope with 2 avalanche photodiode detectors for dual-channel 2D STED (orange, dark red) or conventional confocal imaging of 3 dyes (green, orange and dark red). Piezo-driven z-focus for 3D acquisition, manual XY stage for standard slides. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.723) |
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| {width="6cm"} | Upright point scanning microscope with 2 avalanche photodiode detectors for dual-channel 2D STED (orange, dark red) or conventional confocal imaging of 3 dyes (green, orange and dark red). Piezo-driven z-focus for 3D acquisition, manual XY stage for standard slides. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.723) |
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### Live Spinning Disk
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| {width="1200"} | Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318). |
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| {width="6cm"} | Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318). |
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### Spinning Disk 1
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| {width="600px"} | Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223) |
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| {width="6cm"} | Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223) |
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### LSM-980
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| {width="600px"} | Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives. |
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| {width="6cm"} | Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives. |
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### LSM-700
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| {width="600px"} | Installed: July 2014. Inverse point scanning confocal microscope with 2 PMT channels. Variable dichroic crystal configuration also allowing (slow) spectral imaging), transmitted light PMT and motorized stage. Widefield illumination: halogen (transmitted) and CoolLed pE-2 LED (reflected). ZEN 2012 acquisition software including "tiling & stitching" macro for larger overview imaging. Environmental control (stage-based) possible upon request. |
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| {width="6cm"} | Installed: July 2014. Inverse point scanning confocal microscope with 2 PMT channels. Variable dichroic crystal configuration also allowing (slow) spectral imaging), transmitted light PMT and motorized stage. Widefield illumination: halogen (transmitted) and CoolLed pE-2 LED (reflected). ZEN 2012 acquisition software including "tiling & stitching" macro for larger overview imaging. Environmental control (stage-based) possible upon request. |
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### Celldiscoverer 7
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| {width="600px"} | System for high-throughput live imaging and screening. For multiwell plates (glass or plastic bottom), dishes and chamber slides. Hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), sCMOS camera, 7-color LED for fluorescence, IR-LED for transmitted light and phase gradient contrast, temperature/CO2 control. Python interface for real-time image analysis and custom workflows (room 1.320) |
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| {width="6cm"} | System for high-throughput live imaging and screening. For multiwell plates (glass or plastic bottom), dishes and chamber slides. Hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), sCMOS camera, 7-color LED for fluorescence, IR-LED for transmitted light and phase gradient contrast, temperature/CO2 control. Python interface for real-time image analysis and custom workflows (room 1.320) |
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### Olympus CellSens
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| {width="600px"} | Inverse widefield microscope unit with solid state LED light source and filter sets to image all conventional dyes. 3-color TIRF and single-point 405 nm FRAP option. Equipped with a 4 Megapixel sCMOS camera for fast high-resolution imaging, sensitive EM-CCD for TIRF and low-light applications, or color CMOS camera. Motorized stage for 5D-data acquisition, full environmental control for long-term live imaging. Microfluidics device for mammalian cells, yeast and bacteria (room 1.223) |
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| {width="6cm"} | Inverse widefield microscope unit with solid state LED light source and filter sets to image all conventional dyes. 3-color TIRF and single-point 405 nm FRAP option. Equipped with a 4 Megapixel sCMOS camera for fast high-resolution imaging, sensitive EM-CCD for TIRF and low-light applications, or color CMOS camera. Motorized stage for 5D-data acquisition, full environmental control for long-term live imaging. Microfluidics device for mammalian cells, yeast and bacteria (room 1.223) |
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# Applicability of Microscopes
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