home.md

@@ -19,52 +19,51 @@ You will find guides for startup/shutdown, how to set up incubation and environm
   
 The Wiki is continuously expanded and updated, so if you have any input, find errors, or have suggestions for material you are missing send us an E-Mail via the usual channels. 
 
-
-
 ## Our Microscopes
 
 ##### STED
 
 Upright point scanning microscope with 2 avalanche photodiode detectors for dual-channel 2D STED (orange, dark red) or conventional confocal imaging of 3 dyes (green, orange and dark red). Piezo-driven z-focus for 3D acquisition, manual XY stage for standard slides. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.723)
 
-### Spinning-Disk Systems
 
-##### Spinning-Disk I
+##### Elyra 7
 
-Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223)
+Inverse super-resolution microscope for lattice SIM, 3D SMLM (PALM/STORM), TIRF and laser widefield imaging. Apotome mode for optical sectioning of thick specimen. Dual-camera port with 2 sCMOS cameras for fast acquisitions. Lasers to image dyes from blue to far red. Multi-positioning (Piezo-driven XY stage with Z-Piezo insert) and hardware autofocus. Environmental box for live imaging with temperature and CO2 control. Widefield illumination: Excelitas Xylis (white LED), halogen lamp. Processing PC for offline image processing (room 1.723)
 
-##### Live-Spinning-Disk
+##### Celldiscoverer 7 
 
-Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318).
+System for high-throughput live imaging and screening. For multiwell plates (glass or plastic bottom), dishes and chamber slides. Hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), sCMOS camera, 7-color LED for fluorescence, IR-LED for transmitted light and phase gradient contrast, temperature/CO2 control. Python interface for real-time image analysis and custom workflows (room 1.320)
 
-### Confocal Microscopes
+##### Olympus CellSens
 
-##### LSM-700
+Inverse widefield microscope unit with solid state LED light source and filter sets to image all conventional dyes. 3-color TIRF and single-point 405 nm FRAP option. Equipped with a 4 Megapixel sCMOS camera for fast high-resolution imaging, sensitive EM-CCD for TIRF and low-light applications, or color CMOS camera. Motorized stage for 5D-data acquisition, full environmental control for long-term live imaging. Microfluidics device for mammalian cells, yeast and bacteria (room 1.223)
 
-Inverse confocal microscope with 2 PMT channels (up to 2048 x 2048 pixels), transmitted light PMT and motorized stage. Spectral imaging via sequential acquisition. Multipositioning, tiling and online stitching. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.223)
 
+##### DeltaVision Ultra
 
-##### LSM-980
+Inverse widefield microscope in Sedat setup equipped with a 4-Megapixel sCMOS camera for fast fluorescence and brightfield imaging. 7-color solid state light source for fluorescence imaging with Köhler and critical illumination, respectively, white LED for brightfield, DIC optics. Motorized XY stage, hardware autofocus and environmental chamber for temperature and CO2 control, onboard deconvolution (room 1.320)
 
-Inverse confocal microscope with up to 6 channels (2 PMT, 4 GaAsP, up to 8192 x 8192 pixels). Airyscan 2 detector (32 channel GaAsP) for super-resolution imaging or high-speed acquisition (Multiplex mode with 4x or 8x parallelisation of detection), transmitted light PMT. FCS option and spectral imaging (via sequential acquisition). Motorized XY stage with Z-Piezo top plate, stage-top environmental control (temperature/CO2) and Python interface for complex workflows. Widefield illumination: 4-channel Zeiss Colibri LED, halogen lamp (room 1.223)
 
-##### Elyra 7
+### Spinning-Disk Systems
 
-Inverse super-resolution microscope for lattice SIM, 3D SMLM (PALM/STORM), TIRF and laser widefield imaging. Apotome mode for optical sectioning of thick specimen. Dual-camera port with 2 sCMOS cameras for fast acquisitions. Lasers to image dyes from blue to far red. Multi-positioning (Piezo-driven XY stage with Z-Piezo insert) and hardware autofocus. Environmental box for live imaging with temperature and CO2 control. Widefield illumination: Excelitas Xylis (white LED), halogen lamp. Processing PC for offline image processing (room 1.723)
+##### Spinning-Disk I
 
-##### Celldiscoverer 7 
+Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223)
 
-System for high-throughput live imaging and screening. For multiwell plates (glass or plastic bottom), dishes and chamber slides. Hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), sCMOS camera, 7-color LED for fluorescence, IR-LED for transmitted light and phase gradient contrast, temperature/CO2 control. Python interface for real-time image analysis and custom workflows (room 1.320)
+##### Live-Spinning-Disk
 
-##### Olympus CellSens
+Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318).
 
-Inverse widefield microscope unit with solid state LED light source and filter sets to image all conventional dyes. 3-color TIRF and single-point 405 nm FRAP option. Equipped with a 4 Megapixel sCMOS camera for fast high-resolution imaging, sensitive EM-CCD for TIRF and low-light applications, or color CMOS camera. Motorized stage for 5D-data acquisition, full environmental control for long-term live imaging. Microfluidics device for mammalian cells, yeast and bacteria (room 1.223)
+### Confocal Microscopes
 
+##### LSM-700
 
-##### DeltaVision Ultra
+Inverse confocal microscope with 2 PMT channels (up to 2048 x 2048 pixels), transmitted light PMT and motorized stage. Spectral imaging via sequential acquisition. Multipositioning, tiling and online stitching. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.223)
 
-Inverse widefield microscope in Sedat setup equipped with a 4-Megapixel sCMOS camera for fast fluorescence and brightfield imaging. 7-color solid state light source for fluorescence imaging with Köhler and critical illumination, respectively, white LED for brightfield, DIC optics. Motorized XY stage, hardware autofocus and environmental chamber for temperature and CO2 control, onboard deconvolution (room 1.320)
 
+##### LSM-980
+
+Inverse confocal microscope with up to 6 channels (2 PMT, 4 GaAsP, up to 8192 x 8192 pixels). Airyscan 2 detector (32 channel GaAsP) for super-resolution imaging or high-speed acquisition (Multiplex mode with 4x or 8x parallelisation of detection), transmitted light PMT. FCS option and spectral imaging (via sequential acquisition). Motorized XY stage with Z-Piezo top plate, stage-top environmental control (temperature/CO2) and Python interface for complex workflows. Widefield illumination: 4-channel Zeiss Colibri LED, halogen lamp (room 1.223)
   
 # Applicability of Microscopes
 | usage                   | STED     | Live-Spinning-Disk | Spinning-Disk-I | LSM-980  | LSM-900  | LSM-700  | Elyra-7 | CD-7 | CellSens | DV Ultra |