| ... | ... | @@ -57,6 +57,7 @@ Installed: July 2014. Inverse point scanning confocal microscope with 2 PMT chan |
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| {width="500"} | :heavy_check_mark: Optical Sectioning <br> :heavy_check_mark: Routine confocal imaging <br> <br> :x: Live Imaging <br> |
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## Super-resolution Microscopes
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### [Elyra 7](Instruments/Zeiss Elyra 7)
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| ... | ... | @@ -65,6 +66,14 @@ Inverse super-resolution microscope for lattice SIM, 3D SMLM (PALM/STORM), TIRF |
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### [STED](Instruments/Abberior STEDYCON)
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Upright point scanning microscope with 2 avalanche photodiode detectors for dual-channel 2D STED (orange, dark red) or conventional confocal imaging of 3 dyes (green, orange and dark red). Piezo-driven z-focus for 3D acquisition, manual XY stage for standard slides. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.723)
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| {width="500"} | :heavy_check_mark: Superresolution Imaging <br> <br> :warning: Particular sample preparation <br> :warning: Photobleaching <br> <br> :x: 3D-Imaging |
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### [Celldiscoverer 7](Instruments/Zeiss Celldiscoverer 7)
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System for high-throughput live imaging and screening. For multiwell plates (glass or plastic bottom), dishes and chamber slides. Hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), sCMOS camera, 7-color LED for fluorescence, IR-LED for transmitted light and phase gradient contrast, temperature/CO2 control. Python interface for real-time image analysis and custom workflows (room 1.320)
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| ... | ... | @@ -74,12 +83,6 @@ System for high-throughput live imaging and screening. For multiwell plates (gla |
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### [STED](Instruments/Abberior STEDYCON)
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Upright point scanning microscope with 2 avalanche photodiode detectors for dual-channel 2D STED (orange, dark red) or conventional confocal imaging of 3 dyes (green, orange and dark red). Piezo-driven z-focus for 3D acquisition, manual XY stage for standard slides. Widefield illumination: halogen lamp, CoolLED pE-2 (room 1.723)
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| {width="500"} | :heavy_check_mark: Superresolution Imaging <br> <br> :warning: Particular sample preparation <br> :warning: Photobleaching <br> <br> :x: 3D-Imaging |
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### [Live Spinning Disk](Instruments/Live Spinning Disk)
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| ... | ... | @@ -88,6 +91,7 @@ Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo- |
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| {width="500"} | :heavy_check_mark: Acquisition speed <br> :heavy_check_mark: Live-Imaging <br> :heavy_check_mark: Versatility - tandem SD system <br> :heavy_check_mark: Large ROIs <br> :heavy_check_mark: FRAP <br> :heavy_check_mark: Nanodissection <br> <br> :warning: fixed pixel size|
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### [Spinning Disk 1](Instruments/Spinning Disk 1)
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Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223)
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