... | ... | @@ -20,9 +20,22 @@ Use a yellow marker pen slide with a coverslip and a slide with beads (100nm Tet |
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2. Image Setup for camera 2 (TV2) calibration: lattice SIM, LBF 405/488/561/642, LP560, use the biggest (42µm) grid
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3. Focus on the lattice pattern and take a ‘Snap’
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4. Go to ‘Graphics’ and draw an arrow/line across the pattern
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5. Make sure that the ends of the arrow are placed in the same line across the image, switch off the interpolation
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6. In ‘Graphics’ activate ‘M’ (measurement) and check the angle
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7. Go to ‘Maintain’, and drag out the ‘Dual Camera Adapter Alignment’ tool
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8. Tick 'Calibration pattern'
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8. Change the ‘Rot [°]’ value. Take another snap of the marker sample and measure the angle again, change the ‘Rot [°]’ value again. Repeat until you measure a value lower than 0.05°. Store current position in the ‘Dual Camera Alignment’ tool
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