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## Acquisition Setup
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The LSM-980 can be used in various modes, the "standard" confocal mode on the one hand and a variety of imaging modalities using the Airyscan detector. Depending on the modalities selected, setting up your acquisition will vary slightly and different aspects need to be considered.
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In both cases the "Smart Setup" option is a good point to start from.
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<details>
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<summary>
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### `Environmental Control`
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### `Standard Confocal`
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</summary>
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- Stagetop incubation only
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- Controller: Zeiss TempModule S, CO<sub>2</sub> Module S
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If you want plan to use stage-top incubation on the LSM-980, you need to first open (only one turn needed) the valve on the CO<sub>2</sub> bottle itself, then open the respective valve to the microscope.
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Next, assemble the heating unit with the appropriate insert for your sample carrier. There is one insert for regular slides/Ibidi Dishes **(3)** and one for 35mm dishes **(4)**
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</details>
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For assembly, insert the heated sample holder (**3** or **4**) in the holding frame **(1)**.
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Next, click the assembled holding frame with the insert into the stage of the microscope.
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Finally add the heated lid - it should fit exactly over the heated insert. The fully assembled heating unit is shown in **(5)**. The orientation of the insert is principally irrelevant, but the most sensible setup would be with the heating cables leading to the right.
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<details>
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**Be careful when lowering the condenser, as it could be in a too low position, which would lead to the condenser crashing into the heating lid!**
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<summary>
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In the software, move to the "Incubation" Tab (in the right hand panel in ZEN) and tick the heated lid and one of the inserts (the one you use) and the components will be heated to the selected temperature. Finally tick "CO<sub>2</sub> Small V", assuming you need to supply CO<sub>2</sub>.
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### `Airyscan`
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**Inc PM**: lid
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**H Insert P**: insert for slides/ibidi dishes
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**H Insert M**: insert for 35mm dishes
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</summary>
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- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
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- Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
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- MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
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</details>
|
|
|
|
|
|
|
|
The LSM-980 can be used in various modes, the "standard" confocal mode on the one hand and a variety of imaging modalities using the Airyscan detector. Depending on the modalities selected, setting up your acquisition will vary slightly and different aspects need to be considered.
|
|
|
|
If you want to perform an experiment with a live specimen, or your experiment requires heating or CO<sub>2</sub> supply, we have options for short-term incubation during imaging.
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|
|
|
|
|
|
|
In both cases the "Smart Setup" option is a good point to start from.
|
|
|
|
- Stagetop incubation only
|
|
|
|
- Controller: Zeiss TempModule S, CO<sub>2</sub> Module S
|
|
|
|
|
|
|
|
<details>
|
|
|
|
|
|
|
|
<summary>
|
|
|
|
|
|
|
|
### `Standard Confocal`
|
|
|
|
### `Environmental Control`
|
|
|
|
|
|
|
|
</summary>
|
|
|
|
|
|
|
|
</details>
|
|
|
|
If you want plan to use stage-top incubation on the LSM-980, you need to first open (only one turn needed) the valve on the CO<sub>2</sub> bottle itself, then open the respective valve to the microscope.
|
|
|
|
|
|
|
|
Next, assemble the heating unit with the appropriate insert for your sample carrier. There is one insert for regular slides/Ibidi Dishes **(3)** and one for 35mm dishes **(4)**
|
|
|
|
|
|
|
|
<details>
|
|
|
|
For assembly, insert the heated sample holder (**3** or **4**) in the holding frame **(1)**.
|
|
|
|
|
|
|
|
<summary>
|
|
|
|
Next, click the assembled holding frame with the insert into the stage of the microscope.
|
|
|
|
|
|
|
|
### `Airyscan`
|
|
|
|
Finally add the heated lid - it should fit exactly over the heated insert. The fully assembled heating unit is shown in **(5)**. The orientation of the insert is principally irrelevant, but the most sensible setup would be with the heating cables leading to the right.
|
|
|
|
|
|
|
|
</summary>
|
|
|
|
**Be careful when lowering the condenser, as it could be in a too low position, which would lead to the condenser crashing into the heating lid!**
|
|
|
|
|
|
|
|
- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
|
|
|
|
- Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
|
|
|
|
- MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
|
|
|
|
In the software, move to the "Incubation" Tab (in the right hand panel in ZEN) and tick the heated lid and one of the inserts (the one you use) and the components will be heated to the selected temperature. Finally tick "CO<sub>2</sub> Small V", assuming you need to supply CO<sub>2</sub>.
|
|
|
|
|
|
|
|
**Inc PM**: lid
|
|
|
|
**H Insert P**: insert for slides/ibidi dishes
|
|
|
|
**H Insert M**: insert for 35mm dishes
|
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|
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</details>
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