... | ... | @@ -69,11 +69,19 @@ In the software, move to the "Incubation" Tab (in the right hand panel in ZEN) a |
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### Airyscanning
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### Acquisition Setup
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The LSM-980 can be used in various modes, the "standard" confocal mode on the one hand and a variety of imaging modalities using the Airyscan detector. Depending on the modalities selected, setting up your acquisition will vary slightly and different aspects need to be considered.
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#### Standard Confocal
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#### Airyscan
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- 8 bit, fill about 1/3 to ½ of full range (50-125 counts, a bit more with 8Y-MPLX
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- Multichannel Airyscan alignment: 512 x 512 pixels, high speed, optional: line switching
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- MPLX-8Y, CO-8Y: always bidirectional (bidi correction must be selected in Options, autocorrection done from detector signal)
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#### "Regular" Confocal
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## Technical Specifications
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