... | ... | @@ -68,7 +68,9 @@ Upright point scanning microscope with 2 avalanche photodiode detectors for dual |
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### Live Spinning Disk
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| {width="2000"} | Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318). |
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Inverse spinning disk microscope (Nikon Ti2E double-deck stand) for fast pseudo-confocal imaging, preferably for long-term live applications. Fast high-resolution sCMOS or sensitive EM-CCD camera, multi-positioning and full environmental control. Pulsed 355 nm laser for nanodissection, all lasers available for photomanipulation (FRAP, photoactivation). Widefield illumination: 8-color SPECTRA-III LED, white LED for transmitted light (room 1.318).
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| {width="500"} | :heavy_check_mark: Acquisition speed <br> :heavy_check_mark: Live-Imaging <br> :heavy_check_mark: Flexible - two Spinning Disk systems with 2 cameras each |
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### Spinning Disk 1
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| {width="2000"} | Inverse spinning disk microscope unit for fast pseudo-confocal imaging, preferably for live applications at ambient temperature. Fast sCMOS and high-sensitivity EM-CCD camera. Motorized XY stage with Piezo Z-drive and real time controller for fast 3D imaging, point scanner for FRAP/photoactivation with all available laser lines, stage-top temperature control and a CherryTemp heater/cooler for fast temperature shift experiments (10-45°C). Widefield illumination: SpectraX LED lamp, white LED (room 1.223) |
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### LSM-980
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| {width="2000"} | Installed 2020. Inverse point scanning confocal microscope with up to 6 channels (2 alkali PMT, 4 sensitive GaAsP detectors) and the Airyscan 2 detector for multiplexed super-resolution imaging. Spectral imaging (lambda scanning, emission fingerprinting) via sequential acquisition, transmitted light PMT and lasers to image all dyes from DAPI to Cy5. Multi-positioning (Piezo-driven XY stage with Z-Piezo top plate). Biophysical FCS analysis via photon counting modularity possible with dedicated water immersion objectives. |
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